This movie demonstrates the seamless integration of confocal scanning with optical particle manipulation as well as the multiple degrees of freedom offered by JPK’s NanoTracker™ system in positioning a sample relative to a trapped object.
A fluorescent bead is optically trapped and moved in 3D through a sample of fluorescently labeled cells. Movements of the cell sample in x, y and z are performed using a three-axis piezo scanner (when the cells move relative to the field of view or the focus changes). For movements of the bead relative to the camera image, the 3D trap steering system of JPK’s NanoTracker™ is used (when the bead moves in x and y or in and out of focus). The movie nicely shows that the sample can be positioned in 3D relative to a trapped bead and also the bead can be freely moved through the stationary sample.
Post time: Aug-07-2017